Menu: (Note your choice when you register.)

Living cells contain proteins complexed as dynamic structures that control many distinct aspects of cell behavior. Addressing the complexity of protein function requires a comprehensive understanding of subcellular localization and protein stoichiometry.

Many genetic disorders such as Parkinson’s disease, diabetes and Alzheimer’s are a result of faulty protein signaling mechanisms found within cells. Several fluorescent microscopy techniques have been developed to probe intracellular dynamics, including epifluorescence, confocal, and two-photon microscopy.

• Epifluorescence microscopy is a relatively simple imaging technique but is deficient in blocking out of focus light, which limits z-axial resolution.
• Confocal microscopy greatly reduces out of focus light to allow for precise optical sectioning, although, it also results in a reduced signal to noise ratio.
• Two photon microscopy provides deeper tissue penetration depths by reducing out of focus photodamage but has been observed to exhibit increased levels of autofluorescence.

Here, we will present the pros and cons of epifluorescence, confocal, and two photon microscopy in addition to a novel technique that utilizes discrete Fourier Transforms and Phasor analysis to separate fluorescent protein signal from an autofluorescent background. Using phase resolved optical lock in detection (phOLID), signal to noise ratios were maintained as Dronpa-1, Dronpa-2, and Dronpa-3 were successfully differentiated from GFP and autofluorescence.

Location: Wyndham Garden Hotel, I-435 & Metcalf Ave, Overland Park, KS.
Ph: (913) 383-2550.
Dir: Exit I-435 Metcalf Ave N. Go 1/4 km to 107th St. Turn right. Take immed. right @ BP. Go 100 meters; hotel is on your left.

Times: No-Host Social Hour: 5:30 - 6:00 PM
Dinner Hour: 6:30 - 7:30 PM
Program: 7:30 - 8:30PM

Charge: Dinner $25/20* for members & guests, students $15. No charge for presentation.
*The second price reflects your registration discount, if you register by the deadline.
Dinner reservation deadline is noon on June 09.

The cash bar at mealtime now offers added variety to your beverage options:

• House Wine $4.00
• Domestic Beer $3.50
• Imported Beer $4.00
• Bottled Water $2.50
• Soft Drinks $2.00

Register: june @ ieee-kc.org.
Note your name, daytime phone #, meal selection, and the names of those in your party.

Each meeting provides one hour of professional development.

*****************************************************************

** NO-SHOWS WILL BE BILLED FOR UNPAID RESERVATIONS. **